I am new to CellProfiler and I was hoping that someone could give me some tips on a pipeline that I am working on. I aim to calculate the length of tight junctions in an image. In this particular experiment, the cells were stained for Occludin (a tight junction protein). I started the project but I am having trouble identifying the objects in my image (i.e the threadlike structures). I have attached the project to this post. I look forward to your feedback.
occlengthprep.cpproj (402.3 KB)