Hi CellProfiler Team,
I’m trying to merge three greyscale images (DAPI, Cherry and Brightfield) into one composite image, but I keep getting a green background.
I’ve tried using the GrayToColour module (composite), setting DAPI to blue, Cherry to red and Brightfield to grey. I’ve also tried using GrayToColour to merge DAPI and Cherry and then ImageMath to add the merged image to Brightfield, but the result is the same. I found an earlier post on this forum that included a RescaleIntensity step, which I also tried adding in prior to ImageMath, but the background still had a weird green tinge.
Is there a way to merge two coloured images with a grey brightfield image in CellProfiler?
I’m using the 3.0.0 Windows version.