Merging detected centrosomes


#1

I am detecting cell centrosomes. Once I detect them, I would like to have the option to merge some of them depending on the distance between them. I only saw an option to merge objects that are smaller than certain size. In our context, something like the distance between the two closest pixels could work because, unfortunately, there is a high variability in the size of the centrosomes.

Thanks


#2

Hi JGoni,

We do not currently have anything that merges objects based on distance. What you could try to do is lower the automatic threshold (instead of leaving this option as 1, try .8) and manually increase the smoothing (the identify module tells you the smoothing it used, so try something higher). This might help you accomplish your goals.

Mike


#3

Increasing the smoothing is working quite good.

Thank you very much. :smile: