Migration Assay with Eosin and Hematoxylin



I have successfully been using the human cell pipeline to measure fluorescent labeled cells. My lab is not interested in using CellProfiler to count images with eosin and hematoxylin from Boyden chamber assays. I am not sure where to start with images that don’t have separate channels with fluorescent dyes. Can someone point me to a pipeline for starting point and possible modifications I might need to make. Thanks!


If you search the forum archives for those stains (or even just H&E), there are a lot of pipelines already existing; you may find these blog posts handy.

Good luck!