I would like to use CellProfiler to measure cell area, cell shape, and cell volume from z-stacks that are saved as .nd2 files.
I loaded my images by dragging and dropping them into the images module. Then when I use the “protocol” described in the manual (http://cellprofiler.org/manuals/current/) to extract the metadata from these images most things look good. I only wondered if it is alright that the Series column only gives zeros (see printscreen below) I would expect that every file would become a new serie.
When I continue following the manual to the NamesAndTypes module problems arise when selecting Metadata as ‘image set matching method’. I get this error:
Can someone help me solve this problem?
Thanks in advance.
Kind Regards, Hanneke