Hi, I am new to image processing and cell biology. Our task is to detect nuclei and cells in microscopy images from vital hepatocytes (no fluorescence images). A typical example you can find here (original image and 8bit grey values):
I tried some modules by trial and error but with poor results. I suppose that a person, that is familiar with cell imaging, can quite easily recognize, what is a promising approach.
Can you give me some hints how to proceed, please? E.g. what modules/filters are proper for pre-processing the images and what modules and parameters are promising for cell & nucleus detection?
Thanks for your help.