Nuclear localized LacZ stain in counterstained nuclei


Dear All,

Greetings! I am very new to CellProfiler.

I have a 40X BF image, where LacZ stain(blue) is co-localized with neutral red, a nuclear stain. My task is to count the number of cells which have only nuclear localized LacZ stains.

I’d appreciate if someone help me out with a hint on how to proceed. Thanks.