I have a Dorsal Root Ganliga section here from mouse, stained with DAPI. Unlike cells in a dish, some of the nuclei are very close together and have irregular shapes.
When the nuclei are well separated and round, IdentifyPrimaryObjects works just fine. But for clumps with weird shapes, the program is struggling.
here is an example. Does anyone have any tips?
And I’m attaching the original too. Sorry if this is a newbie question.