I have been trying to analyse my image which contains 3 channel (red green blue) Red contains principle cell, and blue contains DAPI nuclei. Green channel is distributed in both in and outside of the blue.
I would like to count the number of cells, number of nuclei. I would also want to measure the intensity inside and outside of the nuclei. I found cell profiler might be the best option. Any suggestion would be highly appreciated.
Thanks in advance.
Queensland Brain Institute
The University of Queensland.