I use DAPI to label nuclei of neural tissue containing glia. The glial nuclei are slightly smaller on average and a lot brighter. I am having a difficult time training CellProfiler to distinguish the two. My interest is mainly in the neurons. Any ideas short of just labeling the cells with NeuN (a neuronal nuclei marker)?
I would be pleased to give out a sample image that I am working with, but I don’t have a website to post it to. I could E-mail it though.