I’m a new user of Cell profiler, and therefore have no expertise in building a suitable pipeline for my needs. I was wondering if anyone might have a pipeline already made for the analysis I need to do, or whether anyone could advise me on which modules to use.
The loss of protein of our interest leads to an increase of autophagosome per cell. We would like to enumerate this change in number and, so that we can then try and reverse the phenotype experimentally using pharmacological approaches able in restoring the activity of our protein.
Many thanks in advance!