Ok, as the tutorial video said, to eliminate bubbles and the edge of the well, if you scan the origRed image you are able to eliminate them after, when you mask the image. But this is what i got:
and when i continue to mask the image with the origGray image, this is what i get:
The only thing i can tell is that playing with the threshold and with the typical diameter of objects in this picture and in others i was able to shape specifically the worms. But the point of this step is not to do this, so i'm really lost, just to be honest.
And with the analyser my idea was to quantify the numer of fat droplets or intensity of sign in each worm, and compare between different strains.
Thanks for everything