**Is CP analyzing pictures in numerical/alphabetical order? **
I can’t find this information in any description or manual, but it’s crucial for my work. I’m getting quite desperate about this…
I’m trying to make a Pipeline for High Troughput Cardiomyocyte Cell size analysis. The pictures are taken with the GE Healthcare Incell Analyzer and the pictures names are for example
A - 1(fld 4 wv DAPI - DAPI).tif
A - 1(fld 4 wv TexasRed - TexasRed).tif
(Meaning picture number 4 from well A-1)
I usually take 25 pictures per well and I want to summarize these 25 pictures per well.
At the moment I want Cellprofiler to analyze a whole 96 well plate and afterwards I split the huge result txt table back to per well using R Graphics.
I tell R Graphics to summarize the first pack of 25 “ImageNumbers” to one well, and then proceed with the next 25.
But if Cell Profiler doesn’t analyze my pictures chronologically for sure, this won’t work.
Is there any module to make sure it will be analyzed in alphabetical/ numeric order ?
Or is there a method to directly summarize all the pictures for well A-1 with CellProfiler?
I’m sorry if this topic was already answered, but I didn’t find any matching results using the search function.
Thank you so much for your help!!!