I’m trying to analyse the following files. The Alexa 546 is an image for cell cytoskeleton, DAPI for the nuclear staining and the FITC is the one for some particles. I am wondering how can I quantify the percentage of cells with FITC labelled particles.
I’m attaching the pipeline that I’m using with this message and I shall be grateful if you could help me modify it inorder to quantify percentage of FITC positive cells.
Nanoparticle_analysisPIPE.mat (1.23 KB)