I’m new to Cellprofiler and I would like to set up a pipeline to measure cell area of cardiac cells in heart tissue using wheat germ agglutinin stain (WGA). I have saw some posts from the this forum and I get a pipeline for this. But, when I download the sample image and run the pipeline, it comes out with no information. The pipeline, the output file, and the sample image are enclosed below, would some one help with this? Many thanks!
By the way, my images are similar with the sample image that only one staining with green color in it.FilteredCellsWGA.txt (489 Bytes)
WGA pipe modified.cpproj (441.2 KB)