Thanks for the help! Basically I want to use this image (DAPI stain) to idenitfy the whole mass as one “object”,
then use this image to identify a GFP region as a separate object,
and then identify the smaller objects in the remaining area between the two objects (the little floating bits outside the main GFP mass) so I can count them.
I’m going to give the masked image method outlined below by Anne and Minh a go and see if that works.