Currently I am analyzing nuclei of plant cells (stained with DAPI) taken in 63x magnification. For whatever reason, there are holes in the nuclei. Subsequently one nucleus is identified as two nuclei leading to artifacts which I want to avoid.
Is there, perhaps in the image processing chapter, a way to virtually fill those holes in the nuclei? Just for a proper segmentation in order to enable an accuarate analysis of the whole nucleus without dividing it in two parts?
I already tried the smooth algorithm using the median filter but I could not solve the described problem.
Thanks for your advise,