Since,nuclear translocation assay gives you distance cytoplasm as well as propagated cytoplasm, I plotted two scatter plots of green florescence in nuclei (y-axis) vs either type of cytoplasm (x-axis).
I have attached the two plots.
There are remarkable differences b/w the two for same measurements except the type of cytoplasm used.
I have two queries in this regard:
- What conclusion can be made from these plots?
- Which type: should we use for further study?
Plz help so that correct conclusions can be drawn out.