Quantifying fluorescence of coronary arteries


Hello everyone,

I’m fairly new to CellProfiler and so far I love it, but I’m trying to understand how to detect a coronary artery using cellprofiler. Usually, I’ve splitting the RBG image into multiple channels and tracing the vessel manually - red fluorescence is for the primary antibody (MOMA2), green is VWF (Endothelial cells), blue is DAPI/Nuclei. I wanted to work out a pipeline that would identify the vessel and measure red intensity of the vessel, but I’m having troubles when measuring pixel range (even after measuring the length of the vessel. Does anyone have suggestion or an example pipeline or suggestions that may be of use?