I was wondering if I could some more detail about exactly what the radial distribution module measurements measure.

Here is what the manual says

1 - Fraction of total stain in an object at a given radius.

2 - Mean fractional intensity at a given radius (Fraction of total

intenstiy normalized by fraction of pixels at a given radius).

For #1, does this mean the fraction of the total area occupied by stain (is there a threshold intensity applied) or does it mean the fraction of the total stain intensity.

For #2 is there a way of calculating back out the total intensity at the given radius?

Could you please list out exactly what calculations are made by the module.

I need something to give me the fraction of the total intensity at a given radius, I am not sure if #1 does this or if it just calculates the area occupied by stain.

Please Help!