I was trying to use cellprofiler to analyze muscle cells with central nuclei. The cell membrane is labeled with WGA and nuclei with DAPI. what I did is to identify the muscle and then shrink it by 8 pixels, i then use relateobjects to find the shrunked muscle cells (parent) with nuclei (children). When I visually examine the images, I saw quite a few cells with obvious nuclei outlines were not classified correctly. could you please help me with this?
Attached is the image that I overlaid the classified cells (red) with shrunkmuscle outlines and nuclei outlines.