I’ve been using the latest release of Cell Profiler to relate speckles to nuclei by using the Identify Primary Objects for Dapi, Secondary for creating the cells and then again Primary for the speckles. I then relate the speckles to the cells using Relate Objects.
Now I would like to do this in 3D, but I understand that the Primary module doesn’t yet exist in for 3D. Is there a way to bypass this and get the measurement a different way?
Thanks a lot for your reply