There’s no official ‘MeasureObjectQuality’ module, but there are definitely ways to determine if any of our hundreds of object measurements can determine in-vs-out of focus for your nuclei:
Easy to set up, but slow and likely less accurate:
Simply go into your existing pipeline , downstream of your IdentifyModule add a bunch of Measurement modules, then either use the WorkspaceViewer (recommended) or DisplayDataOnImage (not as recommended) to look at some candidate measurements- off the top of my head, some of the Intensity Edge measurements, Granularity, Texture, or some of the Shape measurements could all be candidates.
More complicated to set up, but easier and more likely to succeed in the long run:
Create a QC pipeline that includes everything up to and including your nuclear identification, lots and lots of Measurement modules, then ExportToDatabase. You can then use CellProfiler-Analyst’s Classifier tool to actually sort in-focus from out-of-focus nuclei. CPA will then actually tell you which measurements are important for sorting in-focus from out-of-focus nuclei, and you can even save the rules it uses to sort those as a text file. In your original pipeline then, you can add the FilterObjects module (plus whatever measurements turned out to be important) and read that rules text file to apply that filtering to your whole data set. You can take a look at our tutorials page for a video tutorial of doing this in a previous version of Analyst.