I am starting to convert my old pipeline from Cellprofiler2 to Cellprofiler3. I work on organoids and found that the laplacian of gaussian was very useful for declumping…what would people recommend using instead since this feature was removed?
Can you upload one of your 2.2 pipelines and a sample image? It’s hard to know in the abstract, but if you give us specific examples we may be able to help. Thanks!