Replacement for Laplacian of Gaussian


#1

I am starting to convert my old pipeline from Cellprofiler2 to Cellprofiler3. I work on organoids and found that the laplacian of gaussian was very useful for declumping…what would people recommend using instead since this feature was removed?


#2

Can you upload one of your 2.2 pipelines and a sample image? It’s hard to know in the abstract, but if you give us specific examples we may be able to help. Thanks!