Rescale overlay images


#1

Hi,
How can I rescale overlay images I created to increase the contrast? I need to do this to visualize the nuclei that are outlined in the final image. Previously this was possible within the OverlayOutlines module. I have tried to use the RescaleIntensity module but get error messages using many methods, or don’t see improvement.

Thanks!
Katherin


#2

Hi,

You can try to use ImageMath > Operation: Log transform base 2, then multiply the image by something , e.g. 4 or 8 to see what suits you.

Then do Overlay on top of the transformed image.

Hope that helps.


#3

I found another way: I rescaled the Nuclei images and then generated the overlays after rescaling. I was able to see segmented nuclei using the method min and max for each image.
Thanks for your help!