Scratch/Wound Assay Help


#1

Hey,

  I am currently doing research on wound healing and was wondering if anyone has performed a scratch assay before? I tried using the cellprofiler scratch assay module but it did a rather poor job at isolating the wound and determining the area. Are there any other modules which I can download? Also is there another software package that would maybe do the job better? Any suggestions on performing the test or how to measure the wounds accurately? Thanks!

#2

Hi,

The wound healing assay that CellProfiler provides is meant to be used as an example to get you started. So the values for the modules work well for those images, but might need some tweaking for your own.

If you let us know what values you chose as input for the modules for your images, or maybe upload some pictures of the results from CellProfiler, we can assess what might be going wrong and get you moving in the right direction.

Regards,
-Mark