Dear Cellprofiler pros,
I am having huge problem segmenting my pictures with DNA (as primary) and membrane (as secondary) objects.
My membrane staining is not very sharp, however with the resolution limitation of our imaging platforms, this is hard to improve.
I am trying different ways to preprocessing the membrane marker and trying different parameters in the “identify secondary object module” but nothing seems to help much: my membrane label is not well recognized.
I am attaching here the images and the pipeline I used.
Could you help me improve my segmentation?
Sorry for some reason the image files cannot be uploaded directly in here.
I put them under separate links.
segmentation.cppipe (13.2 KB)
https://drive.google.com/file/d/1_2iSpl67yOKuOC7WACdlvRAVxtklZ_x9/view?usp=sharing https://drive.google.com/file/d/1Dhx5NhGONN0QHCgkXF7I0rjTnuuOqYUp/view?usp=sharing https://drive.google.com/file/d/1gA7jkPs9BQjnHL_RI56ZnDHpnGYmGIKk/view?usp=sharing