I’m working on a project with a collaborator right now, and she’d like to use CP to identify S. pombe spores in an image that features both spores (4 nuclei in one cell) and haploid cells (1 nuclei in 1 cell).
She’s integrated both a nuclear and a faint cytosolic marker in the RFP channel. I’m wondering if there’s a way that I can use CP to identify the nuclei (RFP), and then identify spores in the same image by segmenting the cells around the nuclei based on the fainter RFP signal, creating cell outlines that encompass either 1 or 4 nuclei.
Is that possible? So far I’ve only been able to incorrectly segment spores into 4 cells.
Sorry for the poor description, it’s late and it’s Friday!