Splitting channels in multichannel .czi images



I have several .czi images with two channels (DAPI e 488). I want to quantify the intensity of 488, but I don’t know how can I split the channells and select just the 488. Can someone help me?




Hi Maria,
Here is a forum post that should explain this issue, along with a pipeline; I definitely recommend searching through old posts as a good way to start troubleshooting most problems! Good luck,