I have time lapse movies of cells with a nuclear fluorescent marker that divide during the movie. I want to analyze each cell individually over time - that is, the mother and the two daughters. I am having no problems with the segmentation, but am stuck with the tracking.
I am currently using the TrackObjects module which works perfectly for the mother cell, but then after division it assigns the same label to both daughter cells as it originally assigned to the mother cell, so I cannot analyze each daughter separately. What I want is for the mother cell to be labeled ‘1’ until it divides into two daughter cells given the labels ‘2’ and ‘3’, or two daughter cells labeled ‘1’ and ‘2’ would be fine as well.