There is no double that Cellprofiler is amazing and powerful.
I am trying to use the TrackObject module to track cells and measure their GFP expression intensity over time. Because some cells divide, I want to plot the curves like these:
I use the TrackObject module and everything goes well. Then I use matlab to get the following tables:
The problem is, I cannot distinguish each child cell. After cell division, I cannot know which child cell is child cell 1. I guess I need the information in the ParentObjectNumber. However, I totally do not know how to use it.
For example, I attach the TrackObjects_Label in each image, and display their ParentObjectNumber below. Can anyone help tell me how I read the ParentObjectNumber? Thank you!