Transfection efficiency


#1

Hi All,

I need help. I did transfection to the cells using GFP-labeled siRNA. After the transfections, we saw the proteins in the form of "dots"in the transfected cells. I’m wondering how could I count the cells which are transfected, in order to get the transfection efficiency. Thanks in advance.

Ps. we would like to attach the picture but it failed. In which extension is possible to attach the picture in this forum?

Best

Ichwan


#2

Please post a picture on the web somewhere that is publicly accessible, and then provide a link here.