Trouble identifying primary objects - high cell density


#1

Hello,

I have some trouble identifying my primary objects; they are very dense and are difficult to separate based on intensity, or shape - could any of you point me towards the best thresholding setting within the Identify Primary Objects module?
The life database is here:


Thanks a million :slight_smile:


#2

Hi there,

It’s indeed a challenging case. You can try do the following:

Here is the example pipeline: lif.cpproj (643.1 KB)

Good luck.


#3

Hi Minh,

It’s working a treat, thank you!
Kinga