It's indeed a challenging case. You can try do the following:
- UnmixColors to collect the green channel
- Use some combination of Smooth and Mask, to mask the edge of the tissue out, since it's a confusing region.
- Then clean the (remaining) green image a bit : using EnhanceOrSuppressFeatures >> Suppress, feature size 5
- Then IdentifyPrimaryObjects
Here is the example pipeline: lif.cpproj (643.1 KB)