Is there a way to figure out what the absorbance of your stain is, so you can set a custom filter in the “unmix colors”?
I have some liver slides stained with ORO, and on my test run (of a batch of about 5 samples they showed me) unmixing with eosin worked pretty well. The problem is, with this larger batch of samples I have now, There are some where I have a little off-target staining, but the off target is a little more blue. I was hoping I could create a mask to filter out this off-target stain, but to do that, I’d need to figure out the absorbance.
I just thought if any of you had any tips and techniques for figuring out what you want to use for your custom stain numbers, it would be helpful to share with everyone.