I created a pipeline where I identify cells with Hoechst (Primary) and a cytoplasmic GFP (secondary). Then I filter the secondary objects based on size, because some of the cells are too big or too small. Then I would like to classify these filtered secondary objects for their intensity from a third channel in CPA.
I could get CPA to work to show me all objects with Hoechst. Can I tell CPA to just use the filtered secondary objects?
transfer_analysis.cppipe (15.9 KB)