Using Cell Profiler With Videos



I was looking at the worm toolbox option for CellProfiler and I think this may be a great way to analyze my videos of C. elegans. I am able to separate the videos into individual pictures, so I have no issues inputting my data. However, I was wondering if CellProfiler looks at the photos as though they belong together (thus outputting some data at the end of the analysis that pertains to all photos together) or if it only analyzes each individual photo and gives information about each individual picture. I’m really hoping to get some statistics at the end such as velocity, distance travelled, etc.


You can definitely track across frames- you can read more about that in the CP help under “Creating A Project”->“Loading Image Stacks and Movies”. Please let us know once you’ve looked at that if you have specific issues!


Perfect thanks! I’ll let you know if I have any issues. I plan to test it out tomorrow.