Hi guys. I´ve been trying to segment this images for weeks now. However, I´m not being able to get a general pipeline for all of them. The problem is that they have very different signals being some of them very close to background levels. Can anyone, please, take a look to the attached pipeline (v.17, already!) and see if there is any tip that could help me?
I´ve tried already several illumination correction methods and different segmentation protocols.
Attached 5 imagens. I can provide many more, I just can't upload them via the forum.
RPColonyScreenV17.cppipe (23.2 KB)