Vessels in tissue sections



I am new in CellProfiler and I am trying to obtain informations about the blood vessels in immunofluorescent tissue sections. I would like to know the % of area covered (per field), the number of vessels (per field), the diameter of the vessels (on this parameter I have concern mainly for the vessel with a “sling” shape as I don’t know which parameters can gave me the right result between Min Feret Diameter and Minor Axis Length), I would also try to count how many vessels have an elongated shape and how many have a “sling” shape or a hole in the middle. I am sending 2 images as example.

I wrote a pipeline but I also have issues with the pipeline as it doesn’t save the images (even if it says it does).


ValeriaVessels.cpproj (431.8 KB)



Could you please upload your images again. Images should be either 8 or 16 bit.