I am trying to analyse some images from a wound healing assay. The example pipeline and it is good at finding the “tissue” in my control (T0.tiff), but not so good at identifying the cells once they have started to migrate (T24.tiff/T48.tiff/T72.tiff). As these cells move quite independently (rather than holding hands!) perhaps I need to adjust the settings to account for this?
Any advice/tips much appreciated!
Please find my images here: https://drive.google.com/drive/folders/0B9WqsdNBlJLELWppX1BvM1Nxc2s?usp=sharing